Journal: Stem cells and development

Article Title: p38 mitogen activated protein kinase controls two successive-steps during the early mesodermal commitment of embryonic stem cells.

doi: 10.1089/scd.2010.0213

Figure Lengend Snippet: FIG. 7. p38MAPK activity is required for 2 early successive steps in skeletal myogenesis of ES cells. EBs from wt CGR8 ES cells were differentiated in the presence or absence of PD169316 for different periods as indicated schematically in Fig. 6A. EBs were plated at day 7 and analyzed at day 26 of differentiation. (A) The ratio of differentiated EBs with spontaneous contractile myotubes was evaluated for each cell line; at least 50 different EBs per cell line were counted. Histogram shows the means SEM of 3 independent experiments. (B) In these experiments, mRNAs were extracted at day 26 and analyzed by real-time RT-PCR for expression of the specific skeletal muscle marker MyH1. Results are expressed in arbitrary units, with the values of wt CGR8 at day 26 taken as 1, and are the means SEM of at least 3 independent experiments. Significance between control and treated cells is given as *P < 0.05, **P < 0.01, and ***P < 0.001. (C), Differentiated cells at day 12 were analyzed by flow cytometry analysis of Myogenin. A histogram representative of 3 independent experiments is shown. For each experiment, control value (irrelevant) was determined as no more than 5% of positivity. (D) Differentiated cells at day 12 were analyzed by immunofluorescence using anti-MHC antibodies (magnification: 100). Nuclei were stained with DAPI. Color images available online at www.liebertonline.com=scd

Article Snippet: Membranes were incubated with antibodies against either all p38MAPK isoforms, MAPKAPK2, MAPKAPK2phospho (Cell Signalling Technology), ERK2, or HA epitope (Santa Cruz).

Techniques: Activity Assay, Quantitative RT-PCR, Expressing, Marker, Control, Cytometry, Staining

Journal: PLoS ONE

Article Title: Mechanisms of Nifedipine-Downregulated CD40L/sCD40L Signaling in Collagen Stimulated Human Platelets

doi: 10.1371/journal.pone.0127054

Figure Lengend Snippet: Platelets were pretreated with 5 μ M nifedipine or nifedipine combined with various drugs for 3 min followed by addition of collagen for 6 min. The expression of phospho-P38MAPK and total-P38MAPK (A), phospho-ERK1/2 and total ERK1/2 (B), phospho-HSP27 and total-HSP27 (C) were determined. Date were expressed as means ± SEM ( n = 5). *** p < 0.001 compared with collagen alone group; ++ p < 0.01, +++ p < 0.001 compared with respective collagen+nifedipine-treated group.

Article Snippet: The phospho-HSP27, total-HSP27, phospho-p38 mitogen-activated protein kinase (p38MAPK), total-p38MAPK, phospho-ERK1/2, total-ERK1/2 antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Mechanisms of Nifedipine-Downregulated CD40L/sCD40L Signaling in Collagen Stimulated Human Platelets

doi: 10.1371/journal.pone.0127054

Figure Lengend Snippet: Nifedipine initially activates PPAR-β/-γ followed by increased formation of NO/cyclic GMP and down-regulation of p38MAPK/ERK1/2/HSP27 signaling as well as ROS generation, which then attenuates MMP-2 activity and ultimately inhibits sCD40L release from activated platelets.

Article Snippet: The phospho-HSP27, total-HSP27, phospho-p38 mitogen-activated protein kinase (p38MAPK), total-p38MAPK, phospho-ERK1/2, total-ERK1/2 antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Activity Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Wenyang Zhenshuai Granule on the Expression of LncRNA-MiR143HG/miR-143 Regulating ERK5 in H9C2 Cardiomyocytes

doi: 10.1155/2021/6431007

Figure Lengend Snippet: qRT-PCR primers.

Article Snippet: Adriamycin hydrochloride was provided by Shenzhen Wanle Pharmaceutical Co., Ltd. (H44024359), SYBR Green PCR kit was purchased from Shanghai Sixin Biotechnology Co., Ltd. (BL705A), and Wenyang Zhenshuai granules were purchased from the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine Provided (201902), and ERK5 and p-ERK5 antibodies were purchased from Wuhan Boster Bioengineering Co., Ltd. (ab40908, ab5686).

Techniques: Sequencing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Wenyang Zhenshuai Granule on the Expression of LncRNA-MiR143HG/miR-143 Regulating ERK5 in H9C2 Cardiomyocytes

doi: 10.1155/2021/6431007

Figure Lengend Snippet: Comparison of ERK5 expression in cardiomyocytes of various groups. Note: compared with the normal control group, ∗ P < 0.05; compared with the ADR group, # P < 0.05; compared with the ADR + LncRNA-MiR143HG silence + medicated serogroup, + P < 0.05.

Article Snippet: Adriamycin hydrochloride was provided by Shenzhen Wanle Pharmaceutical Co., Ltd. (H44024359), SYBR Green PCR kit was purchased from Shanghai Sixin Biotechnology Co., Ltd. (BL705A), and Wenyang Zhenshuai granules were purchased from the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine Provided (201902), and ERK5 and p-ERK5 antibodies were purchased from Wuhan Boster Bioengineering Co., Ltd. (ab40908, ab5686).

Techniques: Comparison, Expressing, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Wenyang Zhenshuai Granule on the Expression of LncRNA-MiR143HG/miR-143 Regulating ERK5 in H9C2 Cardiomyocytes

doi: 10.1155/2021/6431007

Figure Lengend Snippet: Comparison of the expression of p-ERK5 and ERK5 proteins in cardiomyocytes of each group. (a) Normal control group, (b) LncRNA-MiR143HG overexpression group, (c) LncRNA-MiR143HG silence group, (d) ADR group, (e) ADR + medicated serum group, (f) ADR + LncRNA-MiR143HG overexpression + medicated serogroup, and (g) ADR + LncRNA-MiR143HG silence + medicated serogroup. Compared with the normal control group, ∗ P < 0.05; compared with the ADR group, # P < 0.05; compared with the ADR + LncRNA-MiR143HG silence + medicated serogroup, + P < 0.05.

Article Snippet: Adriamycin hydrochloride was provided by Shenzhen Wanle Pharmaceutical Co., Ltd. (H44024359), SYBR Green PCR kit was purchased from Shanghai Sixin Biotechnology Co., Ltd. (BL705A), and Wenyang Zhenshuai granules were purchased from the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine Provided (201902), and ERK5 and p-ERK5 antibodies were purchased from Wuhan Boster Bioengineering Co., Ltd. (ab40908, ab5686).

Techniques: Comparison, Expressing, Control, Over Expression

Journal: Journal of Inflammation Research

Article Title: Curcumin Alleviates Osteoarthritis Through the p38MAPK Pathway: Network Pharmacological Prediction and Experimental Confirmation

doi: 10.2147/JIR.S459867

Figure Lengend Snippet: Western blot analysis of curcumin regulation of the p38MAPK pathway. ( A – C ) Western blot and quantitative analysis of p38 and p-p38. ( D – F ) Western blot and quantitative analysis of ASK1 and MEKK3. ( G and H ) Western blot images and quantitative analysis of PKCδ and p-PKCδ. ( I – L ) Western blot and quantitative analysis of BCL-2 and caspase-3. (n = 3). * p < 0.05,** p < 0.01,*** p < 0.001.

Article Snippet: Antibodies against Caspase-3, Bcl-2, PKCδ, MEKK3, ASK1, and GAPDH were purchased from Boster (Wuhan, China).

Techniques: Western Blot

Journal: Türk Tarım ve Doğa Bilimleri Dergisi

Article Title: Ashwagandha root extract attenuates inflammation in Oleic acid induced-ALI/ARDS rat model via inhibition of ACE and MAPK signaling pathways

doi: 10.30910/turkjans.1209593

Figure Lengend Snippet: Figure 1. The effects of Ashwagandha on the MPO and MAPK levels on lung in ALI/ARDS. * denotes significant differences between other studied groups and control (*: p<0.05, ***: p<0.001), ε denotes significant differences between other studied groups and the OA group (ε: p<0.05, εε: p<0.01). Abbreviation used: OA: Oleic acid, Ash: Ashwagandha.

Article Snippet: The obtained supernatants were assayed for Myeloperoxidase (MPO), Glutathione (GSH), Superoxide Dismutase (SOD), Mitogen-activated protein kinases (MAPK), Angiotensin-converting enzyme (ACE) (BT LAB Company, China), and Total oxidant status (TOS) (Rel Assay Diagnostics, Gaziantep, Turkey) levels in rat lung tissue by enzyme-linked immunosorbent assay (ELISA) available kits in accordance with the manufacturer’s manuals.

Techniques: Control

Journal: Journal of Dental Sciences

Article Title: Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis

doi: 10.1016/j.jds.2020.07.013

Figure Lengend Snippet: Western blot analyses: phosphor-ERK and total-ERK in human oral cancer cell lines as compared with a primary culture of normal oral mucosa (HOK). Upregulation of phosphor-ERK (normalized to total-ERK) in OECM1 and Ca922 cell lines as compared with HOK, and a slightly decreased expression in SAS as compared with HOK. Results were quantified using densitometric analysis, normalized to the level of β-actin, and expressed as a fold change relative to the normal oral mucosa. Bars represent means ± standard deviation of the mean (∗ P < 0.05). A representative result of three independent experiments is shown.

Article Snippet: Further, samples were analyzed using 10% SDS-PAGE (Sigma-Aldrich) gels, and the proteins were transmitted onto a PVDF membrane (Sigma-Aldrich) using Bio-Rad's transblot with primary antibodies against phosphor-ERK (Boster Biological Technology, CA, USA; Cat. No. P00104; 1:1000) and total-ERK (Boster Biological Technology; Cat. No. P00104; 1:1000), with species specificity for human tissues and an observed molecular weight of 42–44 kDa; and β-actin (Sigma-Aldrich; 1:1000), followed by horseradish peroxidase (HRP)-conjugated secondary antibodies (Sigma-Aldrich; 1:5000).

Techniques: Western Blot, Expressing, Standard Deviation